Scientist - Киев - резюме 7564


Sergey Tcherniuk

Дата обновления: 20.06.2020

Город: Киев

Профессия: Образование и наука

Тип занятости: Не имеет значения

Опыт работы (лет): 15

Образование: Высшее

Текст резюме:

SERGEY TCHERNIUK

5 av. Jean Jaures
7835 Jouy en Josas, France
E-mail: tchernuk@yahoo.com
Skype: sergey_laba
Tel:(+33) 0651468149
SUMMARY/PROFILE:

• Scientific interests: treatment of human diseases and drug discovery
• Strong expertise in virology, molecular, cell biology and protein biochemistry
• Project realisation in cancer research, parasitology, virology and drug discovery
• Main skills: DNA/RNA manipulation, protein expression and purification, chemical biology, statistical analysis
• Theoretical knowledge of GNS, CHIP-seq, NMR, Mass Spectrometry Analysis, Phage Display, CRISPR-CAS9 technology
• Excellent adaptability to new environment, permanent self-learning

SKILLS

Cell biology: In vitro eukaryotic cell culture (plant and animal), 3D cell culture, selection of cell clones, transient and stable transfection, Xenopus leavis extract, cell transfection (transitory and stable), confocal microscopy and time lapse, FACScan, DNA comet assay, in vitro culture of Plasmodium parasites, histology sections (plants, kidney, placenta), isolation of red blood cells, platelets and PBMC from whole blood.
Virology: Virus production in cell culture, virus isolation and purification (phytoviruses, adenoviruses, influenza virus, pneumoviruses), determination of virus titer, immunoelectrophoresis, hemagglutination, complement activation, minigenome replicon system (RSV, influenza), reverse genetics (RSV).
Molecular biology: PCR, qPCR and RT-PCR, cloning (restriction, recombination), directed mutagenesis, construction of modified vectors and viruses.
Biochemistry: protein expression (bacterial expression system, mammalian expression system, Bac-to-Bac expression system and Multibac expression system), protein purification (FPLC: affinity, ion exchange, gel filtration etc.), photo-affinity labeling (PAL), click chemistry, PAGE, denaturing and native gel electrophoresis, Western blot, ELISA, dot blot, protein crystallogenesis (hanging drop technique), protein-protein interaction: pull-down, immunoprecipitation, surface plasmon resonance (Biacore), ultracentrifugation, analysis of enzymatic activity of proteases and kinases, ATPase activity of kinesins, determination of inhibition type (competitive, uncompetitive, non-competitive) of ATPase activity of kinesins, tubulin preparation from the porcine brain, [3H]-colchicine tubulin-binding assay.
Pharmacology: Isobolographic analysis, drug evaluation in vivo (mouse model).
Biophysical techniques: Spectrophotometry (Polarstar, Tecan), spectrofluorimetry (PTI), transmission electron microscopy, impedimetric analysis of cell-cell interaction.
High throughput screening facilities: Bio-Plex multiplex system (detection and quantification of multiple analytes (proteins and peptides, or nucleic acids) in a single sample volume), robotic platform Freedom EVO (Tecan), High Content Screening/Analysis (Cellomics).
Software: MS Office, Photoshop, Kaleidagraph, Graphpad-Prisme, DNA Strider, MetaMorph, Unicorn, ImageJ, ZEN, BD CellQuest, FlowJo, PyMOL.

EXPERIENCE
2016-2020 Research associate in the laboratory of molecular immunology and virology (Janssen Cilag/INRA), Jouy en Josas.
• Development of the new classes of RSV polymerase inhibitors
• Structural and functional study of the L polymerase of RSV
1. Morgan S. A. Gilman, Cheng Liu, Amy Fung, Ishani Behera, Paul Jordan, Peter Rigaux, Nina Ysebaert, Sergey Tcherniuk, Julien Sourimant, Jean-François Eléouët, Priscila utto-Ortiz, Etienne Decroly, Dirk Roymans, Zhinan Jin, Jason S. McLellan (2019). Structure of the Respiratory Syncytial Virus Polymerase Complex Reveals a Tentacular Arrangement of a Viral Phosphoprotein. Cell 2019 Aug 30. pii: S0092-8674(19)30902-X. doi: 10.1016/j.cell.2019.08.014.
2015-2016 Research associate in the Aix Marseille University, faculty of medecine, Marseille, France, Laboratory NORT.
• Study of the Formyl Peptide Receptor2 role during the influenza A virus infection
1. Tcherniuk S, Cenac N, Comte M, Frouard J, Errazuriz-Cerda E, Galabov A, Morange PE, Vergnolle N, Si-Tahar M, Alessi MC, Riteau B. (2016) Formyl peptide receptor 2 plays a deleterious role during influenza A virus infections. J Infect Dis. 214(2): 237-47.
2. Beatrice Riteau, Marie-Christine Alessi, Marjorie Comte, Sergey Tcherniuk. Modulators of FPR2, compositions and uses thereof for modulating influenza virus replication (https://patents.google.com/patent/WO2017162704A1/en
2013-2015 Research associate in the Florida Atlantic University, department of biomedical science, Boca Raton, Florida, USA, laboratory of Malaria research.
• Study of membrane protein PfEMP1 in the context of antimalarial vaccine development
• High throughput analysis of anti-adhesion activity in plasma of children from malaria endemic areas
• Screening and characterization of new antimalarial and anti-adhesion drugs
1. Maria del Pilar Quintana, Gertrude Ecklu-Mensah, Sergey O. Tcherniuk, Sisse Bolm Ditlev, Andrew V Oleinikov, Lars Hviid and Mary Lopez-Pere. (2019) Comprehensive analysis of Fc mediated IgM binding to the Plasmodium falciparum erythrocyte membrane protein 1 family in three parasite clones. Scientific Reports Apr 15;9(1):6050. doi: 10.1038/s41598-019-42585-0.
2. Olga Chesnokov, Jordan Merritt, Sergey O. Tcherniuk, Neta Milman, Andrew V. Oleinikov. (2018) Plasmodium falciparum infected erythrocytes can bind to host receptor integrin through V3 DBL2 domain of PFL2665c PfEMP1 protein. Scientific Reports Dec 14;8(1):17871. doi: 10.1038/s41598-018-36071-2..
3. Tcherniuk SO*, Chesnokova O, Oleinikov IV, Oleinikov AV. (2017) Nicotinamide inhibits the growth of P. falciparum and enhances the antimalarial effect of artemisinin, chloroquine and pyrimethamine. Mol Biochem Parasitol. 216:14-20. (*corresponding author)
4. Tcherniuk SO*, Chesnokova O, Oleinikov IV, Potopalsky AI, Oleinikov AV. (2015) Antimalarial effect of semi-synthetic drug amitozyn. Malaria journal. 14: 425 .(*corresponding author)
5. Tcherniuk SO*, Oleinikov A. (2015) PgP efflux pump decreases the cytostatic effect of CENP-E inhibitor GSK923295. Cancer letters. 361(1): 97-103. (*corresponding author)
2012-2013 Research associate in the Centre for Biochemical and Macromolecular Research/French National Center for Scientific Research, Montpellier, France, laboratory of Biological Chemistry and Nanotechnology for therapy.
• Identification and characterization of novel allosteric inhibitors of Cdk2 through a biosensor-based screening approach
1. Sergey Tcherniuk*, Morgan Pellerano*, Corine Perals*, Thi Nhu Ngoc Van, Elsa Garcin, Florence Mahuteau-Betzer, Marie-Paule Teulade-Fichou, May C. Morris. (2017) Targeting Conformational Activation of CDK2 Kinase. Biotechnology journal. 12(8) doi: 10.1002/biot.201600531. (*these authors contributed equally)
2009-2011 Research associate in the centre for biochemical and macromolecular research/French National Center for Scientific Research, Montpellier, France, laboratory of Cell cycle.
• Study of spatiotemporal regulation of CENP-E kinesin
• Biochemical and pharmacological characterization of new microtubule drugs
1. Tcherniuk S, Deshayes S, Sarli V, Divita G. and Abrieu A. (2011) UA62784 is a Cytotoxic Inhibitor of Microtubule, not CENP-E. Chemistry & biology. 18(5), 631-41.
2008-2009 Research associate in the Institute of Structural Biology/French National Center for Scientific Research, group of Molecular Biophysics, Grenoble.
• Structural and functional study of the viral protein Vpg
• Evaluation of the cytostatic activity of VPg protein in vivo
• Biochemical and pharmacological characterization of semi synthetic drug Amitozyn
1. Hermant B, Potopalskiy AI, Chroboczek J, Tcherniuk SO*. (2013) Amitozyn impairs chromosome segregation and induces apoptosis via mitotic checkpoint activation. PLoS ONE 8(6).(*corresponding author)
2. Anne-Laure Favier, Renata Grzela, Siergiej Tcherniuk, Charlotte Marianna Harsi and Jadwiga Chroboczek. (2012). A novel human membrane protein interacting with short fibre of enteric adenovirus. ISRN Virology. Vol. 2013, Article ID 709734.
3. Tcherniuk S, Fiser AL, Derouazi M, Toussaint B, Wang Y, Wojtal I, Kondo E, Szolajska E, Chroboczek J. (2012) Certain protein transducing agents convert translocated proteins into cell killers. Acta Biochim Pol. 59(3): 433-9.
2002-2008 Postdoctoral fellowship in the Institute of Structural Biology/French National Center for Scientific Research, laboratory of Structural and Functional Virology, group of Molecular Motors and Protein of the Cytoskeleton Grenoble, France.
• Development of ubiquitin-saporin protein for the treatment of prostate cancer.
• Biochemical and structural characterization of new peptidases Otu-1 and Otu-2
• Screening of chemical library for identification of new kinesin inhibitors
• Biochemical and pharmacological characterization of kinesin inhibitors
• Structural and biochemical study of human kinesins and their protein-partners
1. Guillou C, Kozielski F, Labriere C, Gueritte F, Tcherniuk S, Skoufias D, Thal C, Husson HF. Use of derivatives of indoles for the treatment of cancer. Patent n° EP 09 163523.6. CNRS, France. https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2010150211
2. Tcherniuk S, Skoufias DA, Labriere C, Rath O, Gueritte F, Guillou C, Kozielski F. (2010) Relocation of Aurora B and survivin from centromeres to the central spindle impaired by a kinesin-specific MKLP-2 inhibitor. Angew Chem Int Ed Engl. 49(44), 8228-31.
3. Tcherniuk S, van Lis R, Kozielski F, Skoufias DA. (2010) Mutations in the human kinesin Eg5 that confer resistance to monastrol and S-trityl-L-cysteine in tumor derived cell lines. Biochem. Pharmacol. 79(6), 864-72.
4. Tcherniuk S*, Garcia-Saez I*, Kozielski F. (2006) The structure of human neuronal Rab6B in the active and inactive form. Acta Crystallogr D Biol Crystallogr. 62 (Pt 7), 725-33.(*both authors contributed equally)
5. Tcherniuk S.O., Chroboczek J., Balakirev M.Y. (2004) Construction of tumor-specific toxins using ubiquitin fusion technique. Molecular Therapy, 11, 2, 196-204.
6. Max H. Nanao, Sergey O. Tcherniuk, Jadwiga Chroboczek, Otto Dideberg, Andrea Dessen & Maxim Y. Balakirev. (2004) Crystal Structure of Human Otubain 2. EMBO Rep. 5: 777-782.
7. Balakirev M.Y., Tcherniuk S.O., Jaquinod M., Chroboczek J. (2003) Otubains: A new family of cysteine proteases in the ubiquitin pathway. EMBO Rep. 4, 517-522.
1999-2002 Research assistant in the laboratory of Plant Virology, Kyiv National University.
• Identification and characterization of new strains of plant viruses

1. Tcherniuk S.O., Menchova V. A., (2004) Characteristics of new Ilarvirus isolate isolated from Pelargonia zonale plants. Annual Reports of the National University (Kiev) 1, 48-52.
2. Tcherniuk S.O., Beriozkina V. I., (2004) Identification and characterisation of new Tobamovirus isolate of Cimbidium hibridum plants. Plant Defense 2, 35-39.
3. Tcherniuk S.O., Beriozkina V. I., (2004) Identification and characterisation of new Tobamovirus isolate of Cimbidium hibridum plants. Plant Defense 2, 35-39.
1996-2002 PhD Thesis in virology, Kyiv National University.
• Identification and characterization of new strains of plant viruses isolated from wild plants

1. Tcherniuk S.O., Korneev D.Y. (2001) Comparative analysis of the wheat streak mosaic virus variants using induced fluorescence of the corn chlorophyll. Annual Reports of the National University (Kiev) 4, 70-72.
2. Tcherniuk S.O., Kiriatchenko S.S. (2000) Characterisation of the variants of Tobacco mosaic virus in the plants from the national reserve Askanya-Nova. Annual Report of the National University (Kiev) 3, 69-71.
3. Tcherniuk S.O. (1999) Detection of plant viruses under normal ecological conditions in the plants from the national reserve Askanya-Nova. Annual Report of the National University (Kiev) 1, 48-51.
4. Tcherniuk S.O., Korneev D.Y. (1999) Characteristic changes in the fluorescence of the corn chlorophyll during wheat streak mosaic virus infection. Proceedings of the Ukrainian Academy of Agricultural Sciences: Microbiology section 5, 32-34.
5. Mischenko L.T., Boyko A.L., Tcherniuk S.O. (1999) Effect of clinostating on physiological and biochemical characteristics of wheat plants infected with streak mosaic virus. Biopolymers and Cells 15, 319-323.
6. Tcherniuk S.O. (1999) Isolation of a new variant of the wheat streak mosaic virus from Bromis inermis. Plant Defense 9, 7-9.
7. Tcherniuk S.O., Boyko A. L., Korneev D.Y., Mamenko P.M. (1999) Characteristics of UV-induced fluorescence in Titicum aestivum: Correlation with wheat streak mosaic virus infection. Biopolymers and Cells 15, 445-448.
8. Tcherniuk S.O., Boyko A.L. (1998) Virus detection in plants from the national reserve Askanya-Nova. Proceedings of the International Congress “100th Anniversary of the Askanya-Nova national reserve”, p. 33-35.
9. Tcherniuk S.O., (1998) Virus detection in plants from the national reserve Askanya-Nova. Proceedings of the International conference "Bio-resources and viruses" Kiev, p. 107-108.
10. Mischenko L.T., Boyko A.L., Tcherniuk S.O., Silaeva A.M., Yanichevska G.S. (1998) Effect of clinostating on physiological and biochemical characteristics of wheat plants infected with streak mosaic virus. Proceedings of the International conference "Bio-resources and viruses" Kiev, p. 93.

1996 Training in Cell Biology, Institute of Beer Brewing.
• Production of virus-free plants using the microcloning technique
EDUCATION
2018 - Diploma “Clinical research coordinator”, Paris Descartes University, France
2008 - Diploma ”Animal experimentation level1”, University Joseph Fourier, Grenoble, France
2002 - PhD Thesis in virology, Kyiv National University.
1996 - Master degree in virology Kyiv National University.
INTERESTS: science, language-learning, music composition, sport


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